FIG 2.

Numbers of CFU in the lungs of M. tuberculosis-infected guinea pigs treated with different regimens. Numbers of viable bacteria in whole lungs of M. tuberculosis Kurono-infected guinea pigs were counted after treatment with regimen D, RHZ, DRZ, LEtZA, or DLEtZA (D, DLM at 100 mg/kg; R, RIF at 25 mg/kg; H, INH at 25 mg/kg; Z, PZA at 150 mg/kg; L, LVX at 50 mg/kg; Et, ETO at 50 mg/kg; A, AMK at 150 mg/kg) for 4 and 8 weeks at 5 days per week (3 animals per group, except that there was 1 control guinea pig at 0 weeks and 2 control guinea pigs at 8 weeks because of animal deaths). In the DRZ group at 8 weeks, culture dishes (100 ml) of two samples were contaminated. No bacilli were detected in any 10-ml sample plates or in any sample plates of 4-week treatments. We considered that M. tuberculosis was totally eradicated in this treatment group. In the regimen D group after 8 weeks of treatment, there was one sample whose culture dish (100 ml) was contaminated. No bacteria were detected in the serially diluted 10-ml culture plates, the histopathological analysis sections, or the other two animals of that group. Therefore, we concluded that M. tuberculosis was totally killed in this group. Each value represents the mean of results from triplicate samples, and error bars are SDs from three replicates.