FIG 5.

PBP4 protein amounts in strains grown in the presence of antibiotic. (A and B) COLnex (A) and CRB (B) were grown to an OD₆₀₀ of 0.4 (arrow), and then no drug, nafcillin (NAF) at 0.5 μg/ml (COLnex) or 128 μg/ml (CRB), or ceftaroline (CPT) at 0.25 μg/ml (COLnex) or 64 μg/ml (CRB) was added. (C) PBPs in Bocillin-FL-labeled whole-cell lysates were prepared from cells, all of which were collected at the same time, at an OD₆₀₀ of 1.0 for the COLnex strain or the CRB strain. PBPs 1 to 4 are indicated in the left margin. Data are representative of those from one of two independent experiments.