TABLE 2.

Antibacterial activity of lascufloxacin against quinolone-resistant strains

Straina	Mutation		MIC (μg/ml) for:
	GyrA	ParC	Lascufloxacin	Levofloxacin	Garenoxacin	Ciprofloxacin
S. aureus
MS5935	None	None	0.015	0.125	0.015	0.25
MS5935 gyrA mutant	Ser84Leu	None	0.03	0.25	0.06	0.25
MS5935 1st	None	Ser80Phe	0.03	0.5	0.03	2
MS5935 2nd	Ser84Leu	Ser80Phe	0.125	8	2	16
MS5935 3rd	Ser84Leu	Ser80Phe, Glu84Lys	0.5	32	8	32
MS5935 4th	Ser84Leu, Glu88Val	Ser80Phe, Glu84Lys	2	128	32	32
MS5952	None	None	0.015	0.125	0.03	0.25
MS5952 1st	None	Ser80Tyr	0.03	0.5	0.06	2
MS5952 2nd	Ser84Leu	Ser80Tyr	0.06	2	1	8
MS5952 3rd	Ser84Leu	Ser80Tyr, Ala116Val	0.25	16	0.5	32
MR5867	None	None	0.015	0.125	0.015	0.25
MR5867 1st	None	Glu84Lys	0.03	0.5	0.03	2
MR5867 2nd	Ser84Leu	Glu84Lys	0.125	8	0.5	16
MR5867 3rd	Ser84Leu	Glu84Lys, Ser80Phe	0.5	16	4	32
MR5867 4th	Ser84Leu, Glu88Lys	Glu84Lys, Ser80Phe	2	128	32	32
MR6009	None	None	0.008	0.125	0.015	0.25
MR6009 1st	None	Ser80Tyr	0.03	0.5	0.03	2
MR6009 2nd	Glu88Lys	Ser80Tyr	0.125	4	0.25	8
MR6009 3rd	Glu88Lys	Ser80Tyr, Glu84Lys	0.25	8	0.25	32
MR6009 4th	Glu88Lys, Ser84Leu	Ser80Tyr, Glu84Lys	2	128	64	32
S. pneumoniae
IID553	None	None	0.06	1	0.06	1
NF9884	None	Ser79Tyr	0.125	2	0.125	2
CF9842	None	Asp83Asn	0.06	2	0.125	2
SF9863	Ser81Phe	None	0.06	2	0.25	2
GF9821	Ser81Tyr	None	0.125	2	0.25	2
ST9941	Ser81Phe	Ser79Phe	0.5	16	2	64
SN9981	Ser81Phe	Asp83Tyr	0.25	16	1	32
E. coli
KL-16	None	None	0.125	0.03	0.03	0.008
GF4-3	Asp87Tyr	None	0.5	0.125	0.125	0.125
CP4-1	Asp87Asn	None	0.5	0.25	0.125	0.125
SP4-1	Ser83Leu	None	0.5	0.25	0.25	0.125

^aThe mutant strains of S. aureus, S. pneumoniae, and E. coli were sequentially selected from quinolone-susceptible strains.