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. 2015 Jun 20;5(1):67–71. doi: 10.1093/gastro/gov021

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© The Author(s) 2015. Published by Oxford University Press and the Digestive Science Publishing Co. Limited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Histopathology and immunohistochemistry studies. (A) Liver specimens show polygonal tumor cells arranged mainly in a trabecular pattern and areas of glandular formations with abundant eosinophilic cytoplasm and round nuclei (hematoxylin and eosin stain, original magnification × 100). (B) Esophageal specimens show neoplastic hepatocyte-like cells arranged in nests and trabecular pattern with intervening fibrovascular stroma. Adjacent intestinal metaplasia (Barrett’s esophagus) is evident (hematoxylin and eosin stain, original magnification × 100). (C) Neoplastic cells from esophageal tumor were positive for alpha-fetoprotein staining (original magnification × 200). (D) Esophageal specimens show positive staining for glypican-3 (original magnification × 200). (E) Carcinoembryonic antigen (polyclonal) staining of esophageal specimens, although positive, did not show the definite canalicular staining pattern of hepatocellular carcinoma (original magnification × 400). (F) Esophageal specimens were positive for SALL4 staining (original magnification × 200).