Table 2.

Summary of antibody dilutions and conditions used in the immunoblotting analysis of mitochondrial dynamics, mitochondrial biogenesis, and synaptic proteins in N2a cells treated with Aβ42, Mdivi1+Aβ42, Aβ42+Mdivi1 relative to untreated N2a cells

Marker	Primary Antibody – Species and Dilution	Purchased from Company, City & State	Secondary Antibody, Dilution	Purchased from Company, City & State
Drp1	Rabbit Polyclonal 1:500	Novus Biological, Littleton, CO	Donkey Anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ
Fis1	Rabbit Polyclonal 1:500	MBL International Corporation-life. Woburn, MA	Donkey Anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ
Mfn1	Rabbit Polyclonal 1:400	Novus Biological, Littleton, CO	Donkey Anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ -
Mfn2	Rabbit Polyclonal 1:400	Abcam, Cambridge, MA	Donkey Anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ
OPA1	Rabbit Polyclonal 1:500	Novus Biological, Littleton, CO	Donkey anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ
SYN	Rabbit Monoclonal 1:400	Abcam, Cambridge, MA	Donkey Anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ
PSD95	Rabbit Monoclonal 1:300	Abcam, Cambridge, MA	Donkey Anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ
PGC1α	Rabbit Polyclonal 1:500	Novus Biological, Littleton, CO	Donkey Anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ
Nrf1	Rabbit Polyclonal 1:300	Santa Cruz Biotechnology, Inc. Dallas, TX	Donkey Anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ
Nrf2	Rabbit Polyclonal 1:300	Novus Biological, Littleton, CO	Donkey Anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ
TFAM	Rabbit Polyclonal 1:300	Novus Biological, Littleton, CO	Donkey Anti-rabbit HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ
B-actin	Mouse Monoclonal 1:500	Sigma-Aldrich, St Luis, MO	Sheep Anti-mouse HRP 1:10,000	GE Healthcare Amersham, Piscataway, NJ