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. 2017 Mar 15;16(9):841–851. doi: 10.1080/15384101.2017.1304328

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Figure 4. — Morphological change of ADSCs during differentiation with different inductions. After a 2-weeks culture in the different induction mediums. ADSCs changed from squamous, flat fibroblast-like cells to spindle-shaped cells similar to genuine SCs. (A) ADSCs were induced with OFRFS (B, C) cells were induced with OFFS or OSFRBFS, most cells changed to spindle morphology and the parallel aligned cells were clearly seen. (D) Little bipolar spindle-shape cells were seen when induced with SFRBFS. (E) an image of culture from control group. OFRFS, a combination of OECCM, FSK, RA, FGF and serum; OFFS, a combination of OECCM, FSK, FGF and serum; OSFRBFS, a combination of OECCM,SB, FSK, RA, BME, FGF and serum; SFRBFS, a combination of SB, FSK, RA, BME, FGF and serum. Scale bars = 100 μm.