Figure 7.

Functional assays of SCs derived from ADSCs. (A) Representative micrographs illustrated myelination formation in ADSC-derived SCs with DRG neurons. Notably, co-culture of DRG neurons (green for Tuj-1) with ADSCs-derived SCs (red for MBP) for 12 days, differentiated ADSCs processes were aligned to the neuronal axons, and even were wrapped at certain segments. (B) Myelin-formation was assayed in differentiated ADSCs and DRG neuron at low density. (C) ADSCs (red for CD90) do not form myelination with DRG neuron (green for Tuj-1). (D, E, F) Measurement of release of neurotrophic factors in the differentiated ADSCs at indicated induction time. All data are reported as mean ± SEM. *p<0.05 compared with the relevant controls; **p<0.01; and **p<0.001. Scale bars = 200 μm.