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. 2017 Apr 5;16(9):869–878. doi: 10.1080/15384101.2017.1308617

Figure 3.

Figure 3.

Cisplatin-resistant cells have high expression of NEDD4. (A) Real-time RT-PCR assay was conducted to detect the expression of NEDD4 in parental and CNE/DDP NPC cells. *P < 0.05 vs control. (B) Western blotting analysis was performed to detect the expression of NEDD4 in parental and CNE/DDP NPC cells. (C) Western blotting analysis was performed to detect the efficacy of NEDD4 siRNA transfection. (D) Cell morphology was taken by microscopy in CNE/DDP NPC cells transfected with NEDD4 siRNA. (E) Cell attachment and detachment assays were measured in CNE/DDP NPC cells transfected with NEDD4 siRNA. *P < 0.05, **P < 0.01 vs control siRNA. CS: control siRNA; NS: NEDD4 siRNA.