Table 1.
DNA oligos for siRNA production.
| Target Site | DNA templates |
|---|---|
| Elmod2 211–2351 | Oligo1: GGATCCTAATACGACTCACTATAGACAGGTGTATAGCGAACATCATGA2 |
| Oligo2:AATCATGATGTTCGCTATACACCTGTCTATAGTGAGTCGTATTAGGATCC2 | |
| Oligo3: GGATCCTAATACGACTCACTATATCATGATGTTCGCTATACACCTGTC[2] | |
| Oligo4:AAGACAGGTGTATAGCGAACATCATGATATAGTGAGTCGTATTAGGATCC2 | |
| Elmod2 522–5461 | Oligo1: GGATCCTAATACGACTCACTATAGATCAATCTCGTGTATTTCAGTGAA2 |
| Oligo2:AATTCACTGAAATACACGAGATTGATCTATAGTGAGTCGTATTAGGATCC2 | |
| Oligo3: GGATCCTAATACGACTCACTATATTCACTGAAATACACGAGATTGATC2 | |
| Oligo4:AAGATCAATCTCGTGTATTTCAGTGAATATAGTGAGTCGTATTAGGATCC2 | |
| Elmod2 772–7961 | Oligo1: GGATCCTAATACGACTCACTATAGAGGAAGAGCCAGAAAGCATTATGT2 |
| Oligo2:AAACATAATGCTTTCTGGCTCTTCCTCTATAGTGAGTCGTATTAGGATCC2 | |
| Oligo3: GGATCCTAATACGACTCACTATAACATAATGCTTTCTGGCTCTTCCTC2 | |
| Oligo4:AAGAGGAAGAGCCAGAAAGCATTATGTTATAGTGAGTCGTATTAGGATCC2 | |
| Elmod2 850–8741 | Oligo1: GGATCCTAATACGACTCACTATAGATTGCAATGCTGTGCTCACTTTGA22 |
| Oligo2:AATCAAAGTGAGCACAGCATTGCAATCTATAGTGAGTCGTATTAGGATCC2 | |
| Oligo3: GGATCCTAATACGACTCACTATATCAAAGTGAGCACAGCATTGCAATC2 | |
| Oligo4:AAGATTGCAATGCTGTGCTCACTTTGATATAGTGAGTCGTATTAGGATCC2 | |
| Control3 | Oligo1: GGATCCTAATACGACTCACTATACCTACGCCACCAATTTCGTTT2 |
| Oligo2:AAAAACGAAATTGGTGGCGTAGGTATAGTGAGTCGTATTAGGATCC2 | |
| Oligo3: GGATCCTAATACGACTCACTATAAAACGAAATTGGTGGCGTAGG2 | |
| Oligo4:AACCTACGCCACCAATTTCGTTTTATAGTGAGTCGTATTAGGATCC2 |
1
The numbers are the starting and ending position of the target sites in Elmod2 CDS (NM_001170691.1 in NCBI).
2
Two pairs of DNA oligos are needed for each double-stand siRNA. Oligo 2 is complementary with oligo 1 except an “AA” overhang at 5′; Oligo 3 is complementary with oligo 4 except an “AA” overhang at 5′. In each oligo, gene-specific sequences are underlined; other sequences are for recognition and binding by T7 RNA polymerase.
3
Control siRNA does not target to any mRNA sequence in mouse.