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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Biochemistry. 2016 Oct 21;55(43):5989–5999. doi: 10.1021/acs.biochem.6b00890

Figure 3.

Figure 3

Reactions of EFE in the presence of various L-Arg analogues. (A) Structures of the L-Arg derivatives with differences from L-Arg highlighted in red. (B) Concentrations of 2OG remaining or ethylene and succinate produced after the reaction (or the no-enzyme controls) for various L-Arg analogues and a no-analogue control. Solutions (2 mL) containing 0.5 mM 2OG, 0.5 mM L-Arg analogue, 0.2 mM Fe(II), 0.4 mM L-ascorbic acid, and (when present) 252 nM EFE in 10 mM NH4HCO3 buffer, pH 7.5 were incubated at 25 °C for 80 min and terminated with formic acid. Error bars represent standard errors for n = 2.