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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Biochemistry. 2016 Oct 21;55(43):5989–5999. doi: 10.1021/acs.biochem.6b00890

Figure 4.

Figure 4

Activity of EFE with the alternative oxo-acid substrate, 2OA. The remaining substrates and the formation of products are shown for a representative time course during a 2 mL reaction containing 504 nM EFE, 0.3 mM 2OA, 0.5 mM L-Arg, 0.2 mM Fe(II), and 0.4 mM L-ascorbic acid in 10 mM NH4HCO3, pH 7.5, at 25 °C. Error bars represent standard errors for n = 2.