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. 2017 Jan 4;68(5):953–964. doi: 10.1093/jxb/erw487

Table 1.

Primers used for cloning of pSuc2:6xHis-Cyp1, real-time PCR experiments and site-directed mutagenesis. Bold letters indicate the corresponding amino acid codon changed.

Gene Forward Reverse
pSuc2:6xHis-Cyp1 CAAATACGTGAAGGTAGCAGTTGAC ACACCATTTGTAAGGTCCATAAGCT
IAA10 GACTTCTCAAAAGCTTGATCGAGAG TGAAATCTTTCATTCCTTGGACAA
IAA11 AAAGAACAGTTTTAACGGACGTGAA GACTTATCTGCATCCTCCAATGCT
Tubulin GAAAGCCTACCATGAGCAGC CTTTGGCACAACATCACCAC
R62A GGCTCAACCTTCCACGCTGTGATCCCAGGGTT AACCCTGGGATCACAGCGTGGAAGGTTGAGCC
H133Q GGCTCAACGGAAAGCAAGTCGTGTTTGGACAAG CTTGTCCAAACACGACTTGCTTTCCGTTGAGCC
W128A GTACCGCTAAGACTGAGGCGCTCAACGGAAAGCACG CGTGCTTTCCGTTGAGCGCCTCAGTCTTAGCGGTAC