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. 2017 Mar 10;68(7):1425–1440. doi: 10.1093/jxb/erx024

Fig. 7.

Fig. 7.

The presence of ADT5 in the nucleus is affected by the ability to form stromules. To determine if nuclear localization of ADT5 is dependent on stromules, plants were co-infiltrated with TP-ADT2–CFP (A and B) as a control or ADT5–CFP (C) and an empty vector (dark gray), dominant negative myosin XI-2 (dnMyoXI-2; light gray) and myosin XI-K (dnMyoXI-K/GTD; white), respectively. (A) Percentage of chloroplasts having stromules. Chloroplasts were analyzed if they contained any visible TP-ADT2–CFP fluorescence and were determined to have a stromule if the projection was longer than 1 μm. In total 554, 395, and 579 chloroplasts were analyzed from plants transformed with an empty vector, dnMyoXI-2, and dnMyoXI-K/GTD, respectively. (B) Average length of stromules. A total of 166, 93, and 91 stromules were measured from plants transformed with an empty vector, dnMyoXI-2, and dnMyoXI-K/GTD, respectively. (C) Nuclear localization of ADT5–CFP. Cells were analyzed for CFP fluorescence in the nucleus only if any ADT5–CFP fluorescence was detectable. A total of 131, 190, and 358 cells were analyzed from plants transformed with an empty vector, dnMyoXI-2, and dnMyoXI-K/GTD, respectively. Each experiment was performed on three independent occasions. Significant differences (P<0.001) as determined by a one-way ANOVA (multiple comparisons) are indicated by different letters. Averages ± SE of the mean are plotted.