Fig. 8.

Defense activation does not increase peptide transport. Only active TAMRA–flg22 (5 μM) moves to distal tissue in Col when mixed with FAM–scrambled peptide (10 μM). Peptide transport was not observed in fls2 and bak1. Mixed peptides were applied on a filter paper disc placed on one leaf of in vitro-grown plants. (A) Confocal microscopy images of distal orthostichous leaves in red and green channels and a composite of fluorescence channels with DIC are shown. (B) TAMRA and FAM fluorescence was quantified in red and green channels in the same confocal microscopy images of distal orthostichous tissues 1 d after application. Letters indicate significant difference (calculated separately for TAMRA and FAM; P<0.05, ANOVA/Tukey’s test, n=6).