Fig. 11.

Callose deposition in cotyledons in response to elf18 or Pto DC3000. Ten-day-old seedlings were treated for 24 h with 1 µM elf18 (A) or Pto DC3000 (OD=0.02) (B). Photographs of aniline blue-stained cotyledons under UV epifluorescence were quantified with ImageJ. Data shown are mean values±SE (n>10) of relative callose intensities (RIU, relative intensity units) as measured at 24 h after pathogen or PAMP treatment. One-way ANOVA/Holm–Sidak between treated lines samples: a≠b, P<0.03; a≠c, P<0.05.