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qRT-PCR analysis of SA-related gene expression. Adult 4-week-old plants were sprayed with Pto DC3000 or water as a mock control and harvested at 0 h (white bars), 4 h (black bars) and 48 h (2 dpi; gray bars) post-infection. The relative gene expression was normalized to the reference gene, ACTIN2. Results are from three biological and two technical replicates for each experiment. Error bars indicate SE. Values on the Y-axis are given on a log2 scale.
