Table 1.
Antibodies used for immunocytochemistry
| Antigen | Ab type | Target | Source |
|---|---|---|---|
| Complement components | |||
| C1s | mAb | C1s deposits | Dept Biochemistry, Cardiff, UK |
| C3 | Polyclonal | C3 deposits | Dept Biochemistry, Cardiff, UK |
| C4 | Polyclonal | C4 deposits | Dept Biochemistry, Cardiff, UK |
| C5b | Polyclonal | Activated C-component C5, C5b | Dept Biochemistry, Cardiff, UK |
| C6 | Polyclonal | C6 deposits | Dept Biochemistry, Cardiff, UK |
| C7 | Polyclonal | C7 deposits | Dept Biochemistry, Cardiff, UK |
| C8 | Polyclonal | C8 deposits | Dept Biochemistry, Cardiff, UK |
| C9 | Polyclonal | C9 deposits | Dept Biochemistry, Cardiff, UK |
| C9neo | mAb | Activated lytic C-complex | Dept Biochemistry, Cardiff, UK |
| Other cell markers and immunoglobulins | |||
| CD3/CD8 | mAb | T cells | Dako, Glostrup, Denmark |
| CD20 | mAb | B cells | Dako, Glostrup, Denmark |
| GFAP | Polyclonal | Astrocytes | Dako, Glostrup, Denmark |
| IgG | mAb | IgG deposits, plasma cells | Amersham, Buckinghamshire, UK |
| IgM | Polyclonal | IgM | Dako, Glostrup, Denmark |
| KiM1P | mAb | Monocytes, microglia | Dr W. Bruck, Berlin, Germany |
| 27E10 | mAb | Activated macrophages | BMA Biomedicals, Augst, Switzerland |
| MRP 14 | mAb | Activated macrophages | BMA Biomedicals, Augst, Switzerland |
| CCR3 | Polyclonal | Chemokine receptor | Santa Cruz Biotechnology, Santa Cruz, Calif., USA |
| CXCR3 | mAb | Chemokine receptor | Leukosite |
| CCR5 | mAb | Chemokine receptor | R&D Systems, Minneapolis, Minn., USA |
| EG MBP | Polyclonal | Eosinophil granules | Dr Gerry Gleich, Mayo Clinic, Rochester, Minn., USA |
| Myelin proteins | |||
| MBP | mAb | Myelin | Boehringer, Mannheim, Germany |
| PLP | mAb | Myelin | Serotec, Oxford, UK |
| MAG B11F7 | mAb | Myelin | Doberson et al. (1985) |
| MAG D7E10 | mAb | Myelin | |
| MAG | Polyclonal | Myelin | Matthieu et al. (1990) |
| MOG 8–18C5 | mAb | Myelin/oligodendrocytes | Dr S. Piddlesden, Cardiff, UK |
| MOG Y10 | mAb | Myelin/oligodendrocytes | Dr S. Piddlesden, Cardiff, UK |
| MOG Z12 | mAb | Myelin/oligodendrocytes | Dr S. Piddlesden, Cardiff, UK |
| CNPase | mAb | Myelin/oligodendrocytes | Affinity Research Products, UK |
mAb = monoclonal antibody.