Table 2.
Summary of three-way ANOVA (canopy×growth [CO2]×growth temperature) for leaf parameters measured in E. globulus grown at two atmospheric [CO2] (ambient and ambient+240 µl l–1) and two air temperatures (ambient and ambient+3 oC)
Values are means ±SE. Analysis was done with 2–3 biological replicates per treatment and canopy level. There were no significant three-way interactions, and these were not shown. LMA-TNC and Leaf [N]M-TNC were expressed on a structural dry mass basis [i.e. dry mass corrected for TNC (total non-structural carbohydrate) accumulation].
| Parameter | Canopy | Treatments | Statistical significance, P | |||||
|---|---|---|---|---|---|---|---|---|
| aCaT (n=3) |
eCaT (n=2) |
aCeT (n=3) |
eCeT (n=3) |
Canopy Canopy×CO2 |
CO2 Canopy×temp |
Temp Temp×CO2 |
||
| A sat25 | Upper | 11.9 ± 1.2 | 16.3 ± 0.7 | 14.8 ± 2.5 | 16.6 ± 2.2 | 0.359 | 0.042 | 0.350 |
| (µmol m–2 s–1) | Lower | 14.3 ± 2.4 | 17.1 ± 0.7 | 14.6 ± 0.8 | 19.5 ± 3.2 | 0.797 | 0.916 | 0.935 |
| Leaf [N]M | Upper | 15.8 ± 1.9 | 12.2 ± 0.7 | 24.7 ± 3.5 | 18.0 ± 4.0 | 0.959 | 0.025 | 0.0020 |
| (mg g–1) | Lower | 17.4 ± 2.5 | 14.9 ± 0.5 | 22.2 ± 1.9 | 15.7 ± 2.0 | 0.866 | 0.258 | 0.376 |
| Leaf [N]A | Upper | 1.84 ± 0.12 | 1.85 ± 0.27 | 2.32 ± 0.22 | 1.82 ± 0.38 | 0.007 | 0.283 | 0.486 |
| (g m–2) | Lower | 1.47 ± 0.24 | 1.42 ± 0.10 | 1.55 ± 0.13 | 1.35 ± 0.18 | 0.725 | 0.520 | 0.331 |
| PNUE | Upper | 92 ± 15 | 126 ± 23 | 89 ± 11 | 133 ± 15 | 0.000 | 0.000 | 0.444 |
| [µmol (mol N)–1 s-1] | Lower | 136 ± 4 | 171 ± 19 | 133 ± 12 | 201 ± 8 | 0.531 | 0.544 | 0.280 |
| Rubisco sites | Upper | 22.5 ± 1.5 | 18.9 ± 2.2 | 30.5 ± 5.9 | 23.9 ± 2.3 | 0.145 | 0.026 | 0.046 |
| (µmol m–2) | Lower | 18.0 ± 4.3 | 8.8 ± 0.6 | 21.2 ± 4.3 | 15.1 ± 2.2 | 0.427 | 0.345 | 0.904 |
| Soluble proteins | Upper | 5.4 ± 0.8 | 5.7 ± 1.3 | 6.8 ± 0.3 | 6.7 ± 1.9 | 0.059 | 0.330 | 0.323 |
| (g m–2) | Lower | 5.2 ± 0.7 | 3.2 ± 1.0 | 5.1 ± 0.6 | 4.5 ± 0.8 | 0.466 | 0.851 | 0.903 |
| Chlorophyll | Upper | 0.75 ± 0.17 | 0.57 ± 0.05 | 0.88 ± 0.07 | 0.85 ± 0.17 | 0.222 | 0.400 | 0.157 |
| (mmol m–2) | Lower | 0.62 ± 0.15 | 0.63 ± 0.04 | 0.72 ± 0.03 | 0.63 ± 0.11 | 0.723 | 0.395 | 0.874 |
| LMA | Upper | 118 ± 7 | 152 ± 13 | 97 ± 11 | 102 ± 2 | 0.000 | 0.002 | 0.000 |
| (g m–2) | Lower | 84 ± 2 | 95 ± 4 | 70 ± 2 | 86 ± 3 | 0.490 | 0.015 | 0.210 |
| Average daily TNC | Upper | 30 ± 1 | 39 ± 2 | 20 ± 3 | 23 ± 1 | 0.000 | 0.003 | 0.000 |
| (g m–2) | Lower | 17 ± 1 | 23 ± 3 | 15 ± 1 | 16 ± 1 | 0.296 | 0.004 | 0.049 |
| LMA-TNC | Upper | 88 ± 5 | 113 ± 11 | 77 ± 7 | 80 ± 1 | 0.000 | 0.003 | 0.001 |
| (g DM-TNC m–2) | Lower | 66 ± 2 | 72 ± 1 | 54 ± 3 | 70 ± 2 | 0.615 | 0.037 | 0.381 |
| Leaf [N]M-TNC | Upper | 21 ± 3 | 16 ± 1 | 31 ± 4 | 23 ± 5 | 0.837 | 0.023 | 0.031 |
| [mg g (DM-TNC)–1] | Lower | 22 ± 3 | 20 ± 1 | 28 ± 2 | 19 ± 2 | 0.890 | 0.297 | 0.317 |