Table 2.
BDNF and NT-3 Production in Grafted Cells
| Cat | BDNF (ng/mL/24h/106 cells) | NT-3 (ng/mL/24h/106 cells) |
|---|---|---|
| ACUTE-1 | Not detectable | 2.70 |
| ACUTE-2 | 5.23 | 3.37 |
| SUB-CHRONIC-1 | 5.02 | 0.26 |
| SUB-CHRONIC-2 | 0.07 | 8.75 |
| SUB-CHRONIC-3 | 7.18 | 15.26 |
| SUB-CHRONIC-4 | 23.13 | 240.59 |
| SUB-CHRONIC-5 | 0.29 | 4.54 |
| CHRONIC-1 | 5.38 | 81.13 |
| CHRONIC-2 | Cells unavailable | Cells unavailable |
| CHRONIC-3 | 1.57 | 9.5 |
| CHRONIC-4 | Cells unavailable | Cells unavailable |
Brain-derived neurotrophic factor (BDNF) and neurotrophic factor 3 (NT-3) production by the fibroblasts grafted into the spinal cord for each cat. Production varied greatly among batches, but there was no indication of a correlation with the locomotor recovery outcome among cats. In some animals, all the cells prepared were used and none were available to measure neurotrophins production from. Pre-implantation microscopic observation showed all cells expressing the green fluorescent protein (GFP) reporter. “Not detectable” indicates that the observed production was too low to quantify. “Cells unavailable” indicates that no cells remained for analysis after grafting.