Fig. 1.
CRISPR/Cas9 vectors used in this study. Three promoters, 35S, WOX2 and RPS5A, were used to express Cas9. Backbone vectors were from the pFAST-R series and express the red fluorescent protein TagRFP in transgenic seeds. p35S-Cas9 is based on pFAST-R02, while pX2-Cas9 and pKIR1.0 are based on pFAST-R01. An sgRNA cassette amplified by PCR was inserted into the HindIII or SbfI site by restriction enzyme-based cloning or Gibson assembly. LB, left border; BastaR, basta resistance gene; 35sT, 35S terminator; RB, right border; HygR, hygromycin resistance gene; hspT, heat shock protein terminator.