Fig. 3.

Applications of MarpoDB. (A) Confocal images for three different promoter elements predictions tested by promoters fusions reporters. Plasmids contained a _proMpEF1a:mTurquoise2-N7 cassette and promoters were introduced upstream of a Venus-N7 sequence. Gemmae from transformed plants were used for confocal imaging. Scale bars represent 250 microns. (B) Batch retrieval of M. polymorpha transcription factors’ promoters. CDS sequences of all MarpoDB genes were extracted and queried against the Plant Transcription Factor Database. IDs of CDSs with transcription factor prediction were then queried back to MarpoDB to extract the corresponding promoter elements’ sequences. Finally, sequences were recoded to match the standard syntax for type IIS assembly.