Fig. 2.

Correction of predicted insertion site using confirmation sequences. For the confirmation of a predicted insertion site, a position specific primer is generated (A) and the resulting amplicon is sequenced from both directions to generate confirmation sequences (B). These are evaluated using BLAST, and if the evaluation yields a positive result the best confirmation sequence is used to correct the predicted insertion position (C). If there is a significant distance between the original insertion position and the confirmed insertion position, we import the confirmation sequence as FST in addition, to make the insertion better accessible in external public databases (D).