Fig 3. Effect of adiponectin on high glucose-induced apoptosis in NRK-52E cells.

Cells were cultured with the defined concentrations of glucose in the presence or absence of adiponectin for 48 h. Flow cytometry was used to quantify cell apoptosis after Annexin V and PI double staining. (a) Flow cytometry graphical data for cell apoptosis. (b) Histogram showing percentage of early and total cell apoptosis respectively. Data are the means±SD of four independent experiments. ^§P<0.05; ^*P<0.01.