Table 4. List of LAMP primer sets designed for Usutu virus (USUV) and St. Louis encephalitis virus (SLEV).
| Species | Primera | Primer sequence (5’–3’) | Input sequences |
|---|---|---|---|
| SLEV | F3 | GAGCACTTGATGTGGGAG | 8 |
| B3 | CAATGATTGCCGAATCGC | ||
| FIP | CTTCCATCCGTAATCCAACTCATCCTGACTTGTCAGTTGTAGTGC | ||
| BIP | AACACATTTGTTGTTGATGGACCCGAGTGAACACCATGCCAA | ||
| LoopF | CCAGCTTCTTCAGGCGTC | ||
| LoopB | CAAGGAGTGTCCAACAGCA | ||
| USUV | F3 | GCTGCCAATGAATACGGA | 6 |
| B3 | TAGTGGAGGGTAGCCAGA | ||
| FIP | GTGAGAACCACTGTGCTCCCTACCCTCCATGAACGCTT | ||
| BIP | TCAGAATACATCACAACATCTCTGGCGTAGGTTGAACAAAGACCCA | ||
| LoopF | GGTCGCAAATCCAATGCC | ||
| LoopB | TTCAATAAGCGCTCAGGC |
aF3 and B3, forward outer and reverse outer primers for LAMP, respectively;
FIP and BIP, inner LAMP primers; LoopF and LoopR, forward and reverse loop primers.