Table 1.

Trial locations, corn hybrid relative maturity, number of plot replicates, infestation type, injury to Cry3Bb1-expressing corn, feeding pressure category, and yield data collections

Year	Location	RMta	Reps	Infestation sourceb	Injury level to Cry3c-expressing corn roots	Feeding pressured	Yielde
2012	Crawfordsville, IA	106	4	TC	Normal	High	Y
	Nashua, IA	106	4	TC	Unexpected	High	Y
	Dekalb, IL	106	4	TC	Unexpected	High	Y
	Homer, IL	115	4	TC + AI 800	Normal	Moderate	N
	Monmouth, IL	115	4	TC	Normal	Low	Y
	Frankfort, IN	115	3	TC	Normal	Low	Y
	Fowler, IN	115	4	TC + AI 800	Normal	Low	Y
	Colman, SD	96	4	TC	Normal	High	Y
	Milbank, SD	96	4	TC	Normal	High	Y
	Hills, MN	96	4	TC	Unexpected	High	Y
	Harmony, MN	96	4	TC	Normal	Low	Y
	Higginsville, MO	115	4	TC	Normal	Low	Y
	Caroll, NE	106	4	TC	Normal	High	Y
	York, NE	106	3	TC	Normal	Low	Y
2013	Clinton, IA	115	4	TC + AI 400	Unexpected	Low	Y
	Gildden, IA	106	4	TC	Unexpected	Moderate	N
	Nashua, IA	106	4	TC	Unexpected	High	Y
	Rudd, IA	106	4	TC	Unexpected	High	Y
	Walcott, IA	106	4	TC	Normal	Moderate	Y
	Dekalb, IL	106	4	TC	Normal	Moderate	Y
	Lexington, IL	115	4	TC + AI 400	Normal	Moderate	Y
	Prophetstown, IL	115	4	TC	Unexpected	Low	Y
	Fowler, IN	115	4	TC + AI 400	Normal	Moderate	Y
	Peterson, MN	96	4	TC	Unexpected	Moderate	Y
	Rochester, MN	96	4	TC	Unexpected	Moderate	Y
	Springfield, MN	96	4	TC	Unexpected	Moderate	Y
	Beemer, NE	106	4	TC	Normal	Low	N
	Beemer, NE	106	4	TC	Normal	Low	N
	Scottsbluff, NE	96	4	TC	Normal	High	N
	York, NE	96	3	TC	Normal	Moderate	Y
	Colman, SD	96	4	TC	Unexpected	Moderate	Y
2014	Charles City, IA	106	4	TC	Normal	Low	Y
	Clinton, IA	115	4	TC + AI 400	Unexpected	Low	Y
	Rudd, IA	106	4	TC	Unexpected	Moderate	Y
	Walcott, IA	106	4	TC	Normal	Moderate	Y
	Lexington, IL	115	4	TC + AI 400	Unexpected	High	Y
	Wyoming, IL	115	4	TC	Unexpected	Moderate	Y
	Peterson, MN	96	4	TC	Unexpected	High	Y
	Springfield, MN	96	4	TC	Unexpected	Low	Y
	Lanesboro, MN	96	4	TC	Unexpected	High	N
	Scottsbluff, NE	96	4	TC	Normal	High	Y
	Colman, SD	96	4	TC	Unexpected	Low	Y
	Arlington, WI	96	4	TC	Unexpected	High	Y

^aRelative maturity of the corn hybrids planted at each site.

^bTrap crop (TC) consisted of mixed maturity corn planted the previous year or a mix of corn and pumpkins. Artificial infestation (AI) occurred at V2–V5 at 400 or 800 eggs per 0.305 m of row.

^cTrials were categorized post hoc for local rootworm population tolerance to hybrids only expressing Cry3 proteins (either Cry3Bb1 or mCry3A).

^dTrials were assigned one of the three feeding pressure categories based on non-Bt node injury scale (NIS) ratings: high (>2 NIS), moderate (1 to < 2 NIS), or low (<1 NIS).

^eYield was not collected at all locations. Sites with Y = yield collected, sites with N = no yield collected.