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HUVECs were pretreated for 2 hours with the indicated concentrations of chicoric acid (12.5-100 μM), followed by oxLDL for 24 hours. A.,B. For Western blot analyses, a monoclonal anti-p-eNOS (Ser1177) and a monoclonal anti-eNOS antibody (for normalization) were used. The values represent means ± SD error from three separate experiments. # P < 0.05 vs. untreated control; *P < 0.05 compared with oxLDL treatment.
