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. 2017 Mar 18;8(18):29625–29642. doi: 10.18632/oncotarget.16377

Figure 1. Impaired maintenance of HSCs in Il2−/− mice BM.

Figure 1

A. Flow cytometry analysis of BM cells for LSK population in WT and Il2−/− mice. B. Proportion of LSK cells in the BM of WT and Il2−/− mice (n = 20 per group). C. LSK cells distribution in absolute numbers in WT and Il2−/− mice (n = 20 per group). D. Cell size of WT and Il2−/− HSCs as reflected by their FSC distribution pattern. E. SSC distribution pattern of WT and Il2−/− HSCs. F. Mean cell size and granularity of WT and Il2−/− HSCs. G. FSC and SSC profiles of BM Lin−Sca1+c-Kit− cells from WT and Il2−/− mice. H. Profiles of WT and Il2−/− BM Lin−Sca1−c-Kit+ cells according to their FSC and SSC distribution pattern. I. Proportion of Lin−c-Kit+CD48−CD150+ cell population in the BM from WT and Il2−/− mice (n = 5 per group). J. Myeloid, lymphoid and erythroid lineage-specific genes expression in WT, Il2+/− and Il2−/− mice. K. Flow cytometry revealing the distribution of LT-HSCs and ST-HSCs among LSK cells in indicated mice (n = 5 for WT and 4 for Il2−/− mice). L. Population distribution of LT- and ST-HSC in the BM of WT and Il2−/− mice based on Flk2 expression (n = 7 per group). M. Flow cytometry profiles of BM cells in WT and Il2−/− mice according to their FSC and SSC distribution pattern (n = 20 per group). N. Quantification of FSCloSSClo and FSChiSSChi cells in WT and Il2−/− mice. O. RT-PCR analysis of LT- and ST-HSC-specific genes expression in sorted LSK cells from WT and Il2−/− mice. P. Semi-quantitative RT-PCR on WT and Il2−/− LSK cells for critical genes involved in HSC maintenance. Q. Gene expression analysis of TFs and signaling molecules in LSK cells from WT, Il2+/− and Il2−/− mice. R. Cell survival and HSC proliferation-specific genes expression in LSK cells from WT, Il2+/− and Il2−/− mice. Numbers inside each dot plot represent percent respective population, and in histograms represent the mean fluorescence intensity (MFI). Data are representative of 2-5 independent experiments and shown as mean ± s.d., in (B, C & N) ***P < 0.0001, in (F) *P = 0.0389, ***P = 0.0001, in (I) *P = 0.0348, and in (L) ***P = 0.0002, unpaired t-test.