Skip to main content
. 2017 Mar 18;8(18):29625–29642. doi: 10.18632/oncotarget.16377

Figure 2. HSC maintenance in BM depends on IL-2 signaling.

Figure 2

A. Enumeration of CD45.1+ donor-derived LSK cells distribution 5 and 11 weeks post-transfer in the BM of irradiated CD45.2+ WT or Il2−/− recipient mice. B. Number of CD45.1+ donor-derived cells in the thymus, spleen and BM of irradiated CD45.2+ WT or Il2−/− recipient mice 5 weeks after transfer. C. Evaluation of body weight, and D. spleen weight of the recipient mice 5 weeks after cell transfer. E. Flow cytometry profiles of the distribution of various donor-derived mature hematopoietic cell populations in the thymus, spleen and BM of recipient mice. Data are representative of two independent experiments (n = 4 per group/experiment). F. Distribution of CD45.2+ WT or Il2−/− donor-derived LSK cells distribution 4 and 13 weeks post-transfer in the BM of irradiated CD45.1+ WT recipient mice. G. Number of CD45.2+ WT or Il2−/− donor-derived cells in the Thymus, spleen and BM of irradiated CD45.1+ WT recipient mice 4 weeks after transfer. H. Spleen weight of the recipient mice 4 weeks after cell transfer. I. Flow cytometry profiles of the distribution of various CD45.2+ WT or Il2−/− donor-derived mature hematopoietic cell populations in the thymus, spleen and BM of CD45.1+ WT recipient mice. Data are representative of two independent experiments (n = 4 per group/experiment). Numbers inside each FACS plot represent percent respective population. Data are shown as mean ± s.d., in (B) **P = 0.0090, *P = 0.0176, (C) **P = 0.0034, (D) **P = 0.0096, (G) **P = 0.0028, (H) *P = 0.0198, and ns = not significant, unpaired t-test.