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. 2017 Feb 7;8(18):29887–29905. doi: 10.18632/oncotarget.15136

Figure 4. PIGN expression aberration was a marker of leukemic transformation and progression.

Figure 4

A. PIGN protein was progressively lost in an AML patient (M4 and M5) and B. PIGN gene expression was significantly (***p<0.0001) downregulated from pre-treatment (M4) to relapse (M5). Error bars represent standard deviation from the mean fold change in gene expression. C and D. MDS92 and MDS-L shared the same origin. (C) PIGN protein progressively lost from the MDS phase (MDS92 cells) to the leukemic phase (MDS-L cells) and (D) PIGN gene expression was significantly (***p<0.0001) higher in MDS92 cells than in MDS-L cells. E. Similarly, PIGN protein expression was more suppressed in the myeloblastic phase (KG1) comparing to its myeloid derivative (KG1a) but F. no significant (NS) difference in gene expression was observed between the KG1a and KG1cell lines. However, the PIGN gene transcriptions in all of the above-mentioned samples were elevated 2- to 5- fold in comparison with PIGN gene expression in CD34+ cells from healthy individuals. Error bars represent standard deviation from the mean fold change in gene expression. G. This simplified model depicted the loss of PIGN protein with disease progression from a less aggressive disease stage to a more aggressive disease stage.