Figure 2. TPr activation is required for HG-induced inhibition of Akt-eNOS signaling in HMBECs. HMBECs were transfected with either control siRNA or TPr siRNA (100 nmol/L) for 48 h and treated with high glucose (HG, 30 mM) overnight.

Quantitative analysis of (A) eNOS phosphorylation and (B) Akt phosphorylation in total cell lysates was performed western blot. The blot is a representative of three blots obtained from separated experiments. n = 3, *P < 0.05 vs. control siRNA alone.