Figure 2. Macrophage depletion suppressed miR-21 expression in transplanted vessels.

(A) Time-dependent increases in miR-21 expression in the media and adventitia of allografts as compared to sham measured with qPCR. (B) Expression levels of miR-21 in adventitial fibroblasts isolated from sham and allograft vessels at day 14. (C) Macrophage depletion with clodronate significantly reduced the expression levels of miR-21 in the medial and adventitial layers of transplanted vessels at day 14. Data are mean ± SD. *P < 0.05 versus sham or control (Con), unpaired t-test or one-way ANOVA as appropriate, n = 4.