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. 2017 Mar 17;8(18):30199–30216. doi: 10.18632/oncotarget.16320

Figure 2. Gamma-secretase inhibitor (GSI) resistant cells overexpress PIM1 and have increased sensitivity to PIM inhibitors.

Figure 2

(A) Immunoblot analysis of activated intracellular NOTCH1 (NICD), c-MYC, and PIM1 levels in SUP-T1 naive, short-term treated (ST) (5 days of 1 μM of the GSI Compound E), persister (P), (7 weeks of 1μM GSI) cells, and H-SB2 cells. (B) Total RNA extracted from SUP-T1 naïve (N), short-term treated, and persister cells and the relative mRNA expression of genes shown were analyzed by qRT-PCR. (C and D) SUP-T1 naïve and persister cells were treated with LGB/AZD for 72 h and in vitro cell growth was analyzed using XTT assay. The growth of DMSO control cells is considered 100% and percentage cell growth for individual treatment is reported relative to the DMSO. When compared to naïve cells, SUP-T1 persister cells showed significantly (p < 0.05) increased sensitivity to LGB/AZD treatment. XTT and qRT-PCR data shown are the average +/− S.D. of three independent experiments. Statistical comparisons performed using an unpaired 2-tailed Student's t-test.