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. 2017 Mar 31;8(18):30438–30454. doi: 10.18632/oncotarget.16737

Figure 1. A77 1726 increases LC3-II expression.

Figure 1

(A-C) Dose-dependent increase of LC3-II levels. A375 (A), MCF-7 (B), and C2C12 (C) cells were incubated in complete DMEM medium in the absence or presence of the indicated concentrations of A77 1726 for 16 hr. Rapamycin (Rapa) (20 nM) was included as a control. LC3 and actin expression was analyzed by Western blot. (D) A375 cells were incubated in complete DMEM medium in the absence or presence of the indicated concentrations of leflunomide for 16 hr. LC3 and actin expression were analyzed by Western blot. (E) Time-dependent increase of LC3-II lipidation. A375 cells were incubated in the presence of A77 1726 (200 μM) for the indicated time. Cell lysates were analyzed for LC3 and actin levels by Western blot. (F) The effect of bafilomycin and colchicine. A375 cells seeded in 6-well plates were incubated in complete DMEM medium in the absence or presence of bafilomycin (10 or 40 nM) or colchicine (5 μM) for 16 hr. Cell lysates were analyzed for LC3 and actin expression by Western blot.