Figure 2. Induction of autophagosomes by A77 1726.

A375 cells were transfected with the expression vector pmLC3-RFP. The cells were left untreated or treated with A77 1726 (200 μM), rapamycin (20 nM), or leflunomide (200 μM) for 16 hr. Autophagosomes were visualized under a confocal microscope (A). The puncta of autophagosomes were counted under a fluorescence microscope and plotted in a bar graph with statistical analysis (B). **p<0.01, compared to the control.