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Cells were cultured in 3D gelatin scaffolds with Ca2+ (7.5 mM) and/or BMP-2 (2nM) for 24 hours and extracts analysed by Western blot. Data was quantified relative to the levels of α-TUBULIN (n = 3). Differences were considered significant at p values: * p < 0.05,** p < 0.01, and ***p < 0.001.
