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. 2017 May 25;12(5):e0178269. doi: 10.1371/journal.pone.0178269

Fig 8. Effect of STAT1 silencing on phosphorylation of ERK½ and STAT3 in presence/absence of EGF treatment.

Fig 8

STAT1 was silenced in HTR-8/SVneo cells using siRNA as described in Materials and Methods. The naïve cells, scrambled siRNA and STAT1 siRNA transfected cells were treated with EGF (10 ng/ml) for 60 min. Panel A shows densitometric plot of total STAT1 levels as compared to actin in presence or absence of EGF treatment to confirm silencing of STAT1 by Western blot. Panels B, C and D represent densitometric profiles of phosphorylated ERK½ as compared to actin, phosphorylated STAT3 at ser727 and tyr 705 as compared to total STAT3 respectively in naïve cells, scrambled and STAT1 siRNA treated cells in presence or absence of EGF. Panel F show representative blots from one of the three independent experiments. The data is shown as mean ± SEM of three independent experiments and band intensities were normalized with respect to loading control during densitometric analysis. *p < 0.05 with respect to untreated control cells.