Table 1. Primers and fluorescence-labeled oligonuclueotide probes for the three real-time PCR assays.

Target Gene	Primer/Probe Specificity	Primer/Probe Sequence (5'-3')	Amplicon (bp)	Position	Accession Number
16S-tRNAIle region	T. pedis	TTGAAGTACACAAGACGCTC		109–128	AF179255
		CCCCTTCCTTATCAGAGAA	118	226–208
		FAM GTGCTCTAACCAACTGAGCTACAGGC BHQ1		207–182
16S-tRNAIle region	T. phagedenis	GTCTATACTCTTAAAACGATGCGC		87–110	AF179261
		CCCCTTCCTTATCAGAGAA	104	190–172
		FAM GTGCTCTAACCAACTGAGCTACAGGC BHQ1		171–146
flaB2	T. medium	CGATACGCCTGAAACAGC		369–386	EF061271
		TACCGACAACACTCATTTCG	122	490–471
		FAM TGAAGCCATCAAGAAGATCAACAAGCAGCG BHQ1		411–440