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. Author manuscript; available in PMC: 2017 May 25.
Published in final edited form as: Science. 2015 Jul 23;349(6252):1115–1120. doi: 10.1126/science.aac7049

Fig. 4. Loss of MDA5 rescues Adar1E861A/E861Aviability.

Fig. 4

(A) LKS+ cells isolated from Rosa26CreERT2 Adar1fl/+ (Δ/+) and Rosa26CreERT2 Adar1fl/E861A (Δ/E861A) infected with pLKO.1 empty vector, shGFP, or (B) two independent shMDA5 [shMDA5(1) and shMDA5(2)] were cultured for 8 days. Results are mean ± SEM (n = 3). **P < 0.005 and ***P < 0.0005 compared with Δ/+. n.s, not significant. (C) Analysis of apoptosis on day 8. (D) Images of viable E13.5 yolk sac, embryo, and FL of the indicated genotype (all Ifih1−/−). Scale: yolk sac and embryo, 5 mm; FL, 1.6 mm. Representative (E) FACS profiles and (F) numbers of FL erythrocytes at E13.5. (G) E13.5 FL qRT-PCR of ISGs. Results are mean ± SEM (+/+, n = 2; E861A/+, n = 8; E861A/E861A, n = 4). *P < 0.05 compared with Adar1+/+Ifih1−/− controls. (H) Photo of an Adar1E861A/E861A Ifih1−/− mouse and an Adar1E861A/+Ifih1+/− littermate at 26 days of age.