Figure 5.
RGD decorated HPGMA hydrogels affect cell behavior. ASCs cultured within HPGMA, HPGMA - PEGDA and PEGDA hydrogels (20 wt%) as a function of incorporated TAMRA-labeled RGD. The distribution of the TAMRA fluorescent label suggests that RGD was immobilized within hydrogels containing HPGMA. TAMRA clustering at the cell surface in PEDGA hydrogels suggests RGD was not covalently immobilized within the hydrogel network. Cells are stained with Calcein A (green) to account for cell viability. Scale bar 200 μm.