. 2017 May 25;12(5):e0178336. doi: 10.1371/journal.pone.0178336

Table 3. Oligonucleotide sequence of primer and probe used for real-time PCR in this study.

Target gene		Primer sequence(5’ to 3’)	PCR product size(base pair)	Reference
β- actin	F	`GCAAGCAGGAGTACGATGAG`	134	In this study
	R	`GCCATGCCAATCTCATCTCG`
	Probe	`FAM-TTCTAGGCGGACTGTTAGCTGCGTTACAC-BHQ1`
Mmp9	F	`CCCGGATCAAGGATACAGCC`	177	[25]
	R	`GGGCGAGGACCATACAGATG`
	Probe	`HEX-AGTTTGGCCACGCGCTGGGCTTAGAT-BHQ1`
Serpine1	F	`CTGCGAAATTCAGGATGCGG`	191	[25]
	R	`GGGTGAGAAAACCACGTTGC`
	Probe	`FAM-AGACTTTGGAGTGAAGGTGTTTCAGCAGG-BHQ1`
Timp1	F	`TCTGCAACTCCGATGTCGTC`	125	In this study
	R	`CCTCAAGGCACTGAACCCTT`
	Probe	`HEX-GTTCGTGGGGACCGCAGAAGTCAATG-BHQ1`
Hp	F	`CCAAGTACCAGGACGACACC`	131	In this study
	R	`ACCATACTCAGCCACAGCAC`
	Probe	`FAM-ACGACAAGGAAGACGACACCTGGTATGC-BHQ1`
S100a8	F	`ATTTTGGGGAGACCTGGTGG`	124	[25]
	R	`ACGGCGTGGTAATTCCCTTT`
	Probe	`FAM-TAACTCCCTGATTGACGTCTACCACAAG-BHQ1`
S100a9	F	`AGGCTACGGGAAGGGCAG`	134	[25]
	R	`GCTGGCCTCCTGATTAGTGG`
	Probe	`HEX-ATGGAGGTCACGGCCACAGCCAC-BHQ1`
Tfrc	F	`CAAAGTTTCTGCCAGCCCAC`	188	[25]
	R	`AACAGAAAGAGACCGCTGGG`
	Probe	`HEX-TATCGGGACAGCAACTGGATCAGCAAAG-BHQ1`
Defb1	F	`CGAATGGAGGCATCTGTTTG`	110	In this study
	R	`CTTCGCCTTCTTTTACCACGA`
	Probe	`FAM-TGCCCTGGACACATGATACAGATTGGCA-BHQ1`
Defb10	F	`ATCTAAGCTGCTGGGGGAAT`	97	In this study
	R	`CATTTTACTCGGGGCGCTAA`
	Probe	`HEX-GTTTGCTTAACAGGTGCCCTGGAC-BHQ1`