Fig 3.

Continuous peripheral administration of NLP at 100 μg/kg BW/h/day for 7 days reduced food and water intake (mg/g BW; a & b). The black line indicates the last day of wash out period after the IP injection study, and the downward arrow indicates the pump implantation. The anorexigenic nature of NLP was found in days 3 and 4 of 7 days. A significant difference in food intake between NLP and saline treated group was observed in both dark and light phase. Water intake was significantly reduced in NLP treated group during dark phase on day 3 and day 4. All data are represented as means ± SEM with n = 6 rats/group. *P < 0.05; **P < 0.01; ***P<0.001 compared to control.