Table 1. Comparison of the estimated OTU richness and diversity indexes of the 16S rRNA gene libraries for clustering at 97% identity.
Samplea | Shannonb | Raw reads | Clean reads | Chao | ACEc | Simpson | OTUsd |
---|---|---|---|---|---|---|---|
RH1 | 5.330 | 36076 | 35603 | 1276.304 | 1285.612 | 0.015 | 1009 |
BK1 | 5.240 | 35962 | 35587 | 1335.727 | 1337.056 | 0.019 | 978 |
RH2 | 6.228 | 36556 | 36016 | 1490.337 | 1488.857 | 0.004 | 1239 |
BK2 | 5.769 | 36350 | 35880 | 1443.947 | 1428.056 | 0.009 | 1160 |
RH3 | 5.378 | 36956 | 36388 | 1479.634 | 1479.729 | 0.022 | 1167 |
BK3 | 5.495 | 37005 | 36492 | 1322.793 | 1335.411 | 0.015 | 1103 |
a Sample names 1, 2 and 3 indicated samples collected after maize had grown for 14d, 35d, and 63d, respectively. RH and BK represent rhizosphere and bulk soil.
b Shannon diversity index calculated using Mothur.
c Abundance-based coverage estimator calculated by Mothur.
d Number of operational taxonomic unit calculated with Mothur.