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. 2017 May 25;13(5):e1006346. doi: 10.1371/journal.ppat.1006346

Fig 5. Characterization of the rSgC binding domain responsible for interaction with the cell surface.

Fig 5

Histograms showing the interaction of purified recombinant M3, full-length rSgC, IgD and R2D with CHO-K1 cells (A) or CHO-618 cells (B). CHO-K1 cells contain GAGs whereas CHO-618 cells are devoid of GAGs. Bound proteins were detected by flow cytometry using an anti His-tag antibody. Light grey histograms represent the signal obtained when no recombinant protein was used. Empty histograms represent the signal obtained with recombinant protein. One representative experiment out of at least three independent experiments is shown.