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. 2017 May 25;13(5):e1006346. doi: 10.1371/journal.ppat.1006346

Fig 6. rSgC and IgD enhance chemokine-dependent migration.

Fig 6

(A) Transwell experiment showing the effect of rSgC or IgD proteins on CXCL12-α-induced migration. A range of chemokine concentrations alone or together with 1:200 molar ratio of chemokine:rSgC or IgD was incubated in the bottom chamber of the transwell during 30 minutes at 37°C in a humidified incubator prior to the addition of Jurkat T cells to the top chamber. Migrated cells were detected in the bottom chamber. Plots show one representative assay performed in triplicate out of at least three independent experiments. Error bars represent standard deviation. (B) Coomassie staining showing a representative purification of the IgD protein used in the chemotaxis experiments. ***P<0.0005.