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. 2017 May 25;13(5):e1006346. doi: 10.1371/journal.ppat.1006346

Fig 7. VZV rSgC enhancement of chemokine activity requires interaction with the chemokine and subsequent signalling through the chemokine receptor.

Fig 7

Transwell experiment showing the effect of pertussis toxin (PTX) (A) or AMD3100 (B) on the chemotaxis of Jurkat T cells towards increasing concentrations of CXCL12-α alone or in the presence of 1:200 molar ratio of chemokine:rSgC. The arrows in (A, B) point to the condition with rSgC only, without chemokine. Transwell experiment showing the migration of THP-1 cells (C, D) towards increasing concentrations of wild type or mutated CCL5 (C) or CCL3 and CCL5 (D) alone or in the presence of 1:200 molar ratio of chemokine:IgD (C) or chemokine:rSgC (D). In all experiments the chemokine was incubated alone or together with VZV rSgC at 37°C in a humidified incubator in the bottom chamber of the transwell prior to the addition of the leukocytes to the top chamber. Migrated cells were detected in the lower chamber at the end of the experiment. Plots show one representative assay performed in triplicate out of at least three independent experiments. Error bars represent standard deviation. *P<0.05; **P<0.005; ***P<0.0005.