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. 2017 May 25;13(5):e1006346. doi: 10.1371/journal.ppat.1006346

Fig 8. Characterization of recombinant VZV lacking gC expression.

Fig 8

(A) Western blots showing expression of gC (left panel), mGFP (middle panel) and gE (right panel) in cell lysates of ARPE-19 cells infected with pOka-WT or pOka-ΔgC-mGFP (VZV WT and VZV ΔgC-mGFP, respectively). The gE and gC panels show the same blot subjected to sequential antibody staining following stripping of the membrane. (B) Western blots showing presence of gC (left panel) or gE (right panel) in cell-free VZV produced from MeWo cells infected with pOka-WT or pOka-ΔgC-mGFP (VZV WT and VZV ΔgC-mGFP, respectively). The two panels show the same blot subjected to sequential antibody staining following stripping of the membrane. (C) Representative electron micrograph of cell-free pOka-WT or pOka-ΔgC-mGFP (VZV WT and VZV ΔgC-mGFP, respectively) subjected to negative staining. Arrows point to enveloped virions, arrowheads to capsids. The magnification bar represents 200 nm.