Fig 4. Examples of different assignment strategies.

Overlapping regions are given in red and locomotion is indicated by arrows. (A) During forward locomotion of Drosophila larvae the association can be done using overlap-based costs and the Hungarian algorithm. (B) Given rolling behavior of Drosophila larvae, an assignment using contour overlaps as costs for the Hungarian algorithm might fail due to inappropriate frame rates. (C) For tracking the snake-like motion of C. elegans, contour-based assignments might be insufficient.