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. 2005 Jan;17(1):37–51. doi: 10.1105/tpc.104.026963

Figure 2.

Figure 2.

DNA Gel Blot Analysis of the S3′ and S4′ Mutants Using an S1-RNase cDNA Probe to Test for Rearrangements in the Regions Flanking the S3- and S4-RNase Genes, Respectively.

(A) EcoRI digest of parents and pollen-part mutants. EF, Emperor Francis (S3S4); Nap, Napoleon (S3S4); 2420, JI 2420 (S4S4′); 2434, JI 2434 (S3′S4); Van (S1S3); ML, Merton Late (S1S4).

(B) HindIII digest of parents and pollen-part mutants. EF, Emperor Francis (S3S4); Nap, Napoleon (S3S4); 2420, JI 2420 (S4S4′); 2434, JI 2434 (S3′S4); Van (S1S3); ML, Merton Late (S1S4).

(C) EcoRI digest of the progeny Van (S1S3) × JI 2434 (S3′S4). Van (S1S3); 2434, JI 2434 (S3′S4); S3S3′ selection; S3S4 selection; S1S3′ selection; S1S4 selection; ML, Merton Late (S1S4).

(D) SstI digest of parents and S3′ mutant. EF, Emperor Francis (S3S4); Nap, Napoleon (S3S4); 2434, JI 2434 (S3′S4); S3S3′ selection; Van (S1S3); ML, Merton Late (S1S4).

Low stringency post-hybridization washes allowed the detection of cross-hybridization of the S1-RNase cDNA probe to both the S3 and S4 alleles.