Figure 4.

Segregation Analysis of the Putative S₄-Haplotype–Specific SFB Sequence to Test Cosegregation with the S₄-RNase Allele.

PCR amplification with specific primers for the putative S₄-specific SFB sequence is shown for genomic DNA of parents and a representative sample of seedlings of the cross Bradbourne Black (S₃S₅) × Merton Late (S₁S₄): BB, Bradbourne Black; ML, Merton Late; 12 seedlings. Presence (+) or absence (−) of the S₄-RNase allele is indicated underneath the lanes. The bands amplified by the internal control primers included in each PCR are indicated with PAL.