Figure 5.
Genomic PCR Amplification of Parents and Pollen-Part Mutants with Specific Primers for the SFB and S-RNase Genes of the S3- and S4-Haplotypes.
The primers used are specific for S3-haplotype–specific SFB (A), S3-RNase (B), S4-haplotype–specific SFB (C), and S4-RNase (D). Samples are as follows: EF, Emperor Francis (S3S4); Nap, Napoleon (S3S4); 2420, JI 2420 (S4S4′); S4′S4′ selection; 2434, JI 2434 (S3′S4); S3S3′ selection; S3′S3′ selection; Van (S1S3); ML, Merton Late (S1S4); water control. The bands amplified by the internal control primers that were included in each PCR are indicated with PAL.